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Gibco™ Rat VEGF-165 Recombinant Protein, PeproTech®

Catalog No. 4003150UG
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Catalog No. Quantity
4003150UG 50 μg
40031100UG 100 μg
4003110UG 10 μg
400311MG 1 mg
40031250UG 250 μg
400312UG 2 μg
40031500UG 500 μg
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Catalog No. 4003150UG Supplier Gibco™ Supplier No. 4003150UG
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Description

Recombinant Protein

Recombinant Rat VEGF 165 is a 38.5 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

Accession Number P16612
For Use With (Application) ELISA, Functional Assay, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 83785
Molecular Weight (g/mol) 38.5 kDa
Name Rat VEGF-165
Quantity 50 μg
Source E. coli
Regulatory Status RUO
Endotoxin Concentration <1 EU/ μg
Gene Alias H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165 b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
Common Name VEGF-165
Gene Symbol VEGFA
Biological Activity Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml
Conjugate Unconjugated
Recombinant Recombinant
Sequence MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Content And Storage -20°C
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 98% by SDS-PAGE gel and HPLC analyses.
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