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  5. Invitrogen™ Rat TIMP-2 ELISA Kit

Invitrogen™ Rat TIMP-2 ELISA Kit

Catalog No. ERA54RBX5
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5 x 96 Tests
96 Tests
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ERA54RBX5 5 x 96 Tests
ERA54RB 96 Tests
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Description

ELISA

Rat TIMP-2 quantitates rat TIMP-2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat TIMP-2.

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Specifications

Accession Number collagenase inhibitor; CSC-21 K; D11Bwg1104e; DDC8; Metalloproteinase inhibitor 2; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; Timp2; TIMP-2; TIMP-2 protein; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2
Assay Range 20 to 5,000 pg/mL
Assay Sensitivity 20 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 29543
Gene Symbol TIMP2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target TIMP2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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