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Rat lung tissue lysate, Non-denatured; Abnova

Catalog No. 89014505
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200 μg
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89014505 200 μg
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Catalog No. 89014505 Supplier Abnova Corporation Supplier No. L037W2
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Description

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Lung
Description Whole tissue lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Rat
Concentration 5 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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