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Invitrogen™ Rat IL-10 ELISA (For Lysates) Kit

ELISA

Supplier:  Invitrogen™ ERA24RBX5

Encompass

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Catalog No. ERA24RBX5


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Description

Description

Rat IL-10 quantitates rat IL-10 in cell lysate, tissue homogenate. The assay will exclusively recognize both natural and recombinant rat IL-10.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
Specifications

Chemical Identifiers

CSIF; cytokine; cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; Interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
10 pg/mL
ELISA
Colorimetric Microplate Reader
IL10
<10
HRP
Cell Lysate 50 μL, Tissue Homogenate 50 μL
IL-10
4 hrs 45 mins
10 to 6,000 pg/mL
HRP
Cell Lysates, Tissue Homogenates
25325
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
1 hrs 20 mins

Specifications

CSIF; cytokine; cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; Interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
10 pg/mL
ELISA
Colorimetric Microplate Reader
IL10
<10
HRP
RUO
2°C to 8°C
1 hrs 20 mins
10 to 6,000 pg/mL
HRP
Cell Lysates, Tissue Homogenates
25325
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
5 x 96 Tests
Cell Lysate 50 μL, Tissue Homogenate 50 μL
IL-10
4 hrs 45 mins
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