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  5. Invitrogen™ Rat CD40 Ligand/TNFSF5 ELISA Kit

Invitrogen™ Rat CD40 Ligand/TNFSF5 ELISA Kit

Catalog No. ERA5RBX5
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5 x 96 Tests
96 Tests
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ERA5RBX5 5 x 96 Tests
ERA5RB 96 Tests
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Description

ELISA

Rat CD40 Ligand/TNFSF5 quantitates rat CD40 Ligand/TNFSF5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat CD40 Ligand/TNFSF5.

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

Specifications

Accession Number Q9Z2V2
Assay Range 0.45-100 ng/mL
Assay Sensitivity 0.45 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Rat
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5;
Gene ID (Entrez) 84349
Gene Symbol CD40LG
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target CD154 (CD40 Ligand)
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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