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  6. Invitrogen™ Rat anti-Mouse IgG2a Secondary Antibody, Biotin, eBioscience™

Invitrogen™ Rat anti-Mouse IgG2a Secondary Antibody, Biotin, eBioscience™

Numéro de catalogue. 501128599
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100 μg
25 μg
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Numéro de catalogue. Quantité
501128599 25 μg
13421082 100 μg
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Numéro de catalogue. 501128599 Fournisseur Invitrogen™ Code fournisseur 13421080

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Description

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies. Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This m2a-15F8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue and by immunocytochemistry on fixed MDCK cells. The antibody can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Spécifications

Antigène Mouse IgG2a
Applications Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone m2a-15F8
Concentration 0.5 mg/mL
Conjugué Biotin
Formule PBS with 0.09% sodium azide; pH 7.2
Espèces hôtes Rat
Méthode de purification Affinity chromatography
Quantité 25 μg
Statut réglementaire RUO
Primaire ou secondaire Secondary
Espèces cibles Mouse
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Secondary Antibody
Forme Liquid
Isotype IgG1 κ
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