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Invitrogen™ Rabbit anti-Sheep IgG (H+L) Secondary Antibody, FITC

Catalog No. PI31627
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1.5 mg
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Catalog No. Quantity
PI31627 1.5 mg
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Catalog No. PI31627 Supplier Invitrogen™ Supplier No. 31627
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Description

Rabbit Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31627 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31627 reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1) Amax = 492 nm; Emax = 520 nm Fluorophore/Protein: 9.1 μg/mg; 3.4 moles FITC per mole IgG Reconstitute with 1.1 mL of distilled water (1.5 mg/mL after restoration). Country of Origin: USA.

Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Sheep IgG (H+L)
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry
Classification Polyclonal
Concentration 1.5 mg/mL
Conjugate FITC
Formulation PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
Host Species Rabbit
Purification Method Antigen affinity chromatography
Quantity 1.5 mg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Sheep
Content And Storage 4°C, store in dark
Product Type Secondary Antibody
Form Lyophilized
Isotype IgG
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