missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Secondary Antibodies
  5. Rabbit Secondary Antibodies
  6. 5000357449

Invitrogen™ Rabbit anti-Mouse IgG2a Recombinant Secondary Antibody, PE

Catalog No. 5000357449
Change view
Click to view available options
Quantity:
500 μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
5000357449 500 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 5000357449 Supplier Invitrogen™ Supplier No. SA510380PE
Only null left

Description

Rabbit Recombinant Monoclonal Secondary Antibody

Rabbit anti-Mouse IgG2a Recombinant Secondary Antibody, PE is specific to Mouse IgG2a with no reactivity towards Mouse IgG1, Mouse IgG2b or Mouse IgG3. It showed no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG2a
Applications Flow Cytometry
Classification Recombinant Monoclonal
Clone 81H27L3
Concentration 0.2 mg/mL
Conjugate PE
Formulation PBS with 5mM sodium azide; pH 7.4
Host Species Rabbit
Immunogen Purified Mouse IgG.
Purification Method Affinity chromatography
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
Show More Show Less