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Invitrogen™ Rabbit anti-Mouse IgG2a Recombinant Secondary Antibody, Alexa Fluor™ Plus 488

Catalog No. 5000331950
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250 μL
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5000331950 250 μL
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Catalog No. 5000331950 Supplier Invitrogen™ Supplier No. SA510380AFP488

Description

Rabbit Recombinant Monoclonal Secondary Antibody

The Rabbit anti-Mouse IgG2a Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is specific to Mouse IgG2a with no reactivity towards Mouse IgG1, Mouse IgG2b or Mouse IgG3. It showed no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. The sensitivity and specificity of each lot is confirmed using ELISA. Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG2a
Applications Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Recombinant Monoclonal
Clone 81H27L3
Concentration 1 mg/mL
Conjugate Alexa Fluor Plus 488
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Host Species Rabbit
Immunogen Purified Mouse IgG.
Purification Method Affinity chromatography
Quantity 250 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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