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Invitrogen™ Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 647

Catalog No. 5000357483
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5000357483 1 mg
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Catalog No. 5000357483 Supplier Invitrogen™ Supplier No. SA510391AF647
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Description

Rabbit Recombinant Superclonal Secondary Antibody

Rabbit anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 647 can detect both Fab2 and Fc region of Mouse IgG. It can also detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b and 3). It has <20% cross reactivity to Human IgG and Rat IgM. It showed minimum to no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG (H+L)
Applications Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Recombinant Superclonal
Concentration 1 mg/mL
Conjugate Alexa Fluor 647
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Host Species Rabbit
Immunogen Purified Mouse IgG.
Purification Method Affinity chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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