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Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Rhodamine

Catalog No. PI31665
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1.5 mg
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Catalog No. Quantity
PI31665 1.5 mg
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Catalog No. PI31665 Supplier Invitrogen™ Supplier No. 31665

Description

Rabbit Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31665 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31665 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulin from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein Absorbance Ratio: A550/A280 = ~ 0.4 (lot-dependent). Reconstitute with 1.1 mL of distilled water (1.5 mg/mL after restoration). Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG (H+L)
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry
Classification Polyclonal
Concentration 1.5 mg/mL
Conjugate Rhodamine
Formulation PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
Host Species Rabbit
Purification Method Affinity chromatography
Quantity 1.5 mg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C
Product Type Secondary Antibody
Form Lyophilized
Isotype IgG
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