Invitrogen™ Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 488

Description

The Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™488 specifically detects the heavy and the light chains of goat IgG. It has ~20% cross reactivity to Hamster IgG and showed minimum to no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. The sensitivity and specificity of each lot is confirmed using ELISA. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.