missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. RNA Isolation and Purification Products
  5. Invitrogen™ Qubit™ microRNA Assay Kits

Invitrogen™ Qubit™ microRNA Assay Kits

Catalog No. Q32881
Encompass
Change view
Click to view available options
No. of Reactions:
100 Reactions
500 Reactions
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. No. of Reactions
Q32881 500 Reactions
Q32880 100 Reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. Q32881 Supplier Invitrogen™ Supplier No. Q32881
Only null left

Description

Achieve accurate miRNA quantitation, even in the presence of rRNA, large mRNA, or contaminants, with the Qubit microRNA Assay Kit, which offers 100 and 500 assay formats.

Achieve accurate and precise quantification of small RNA (∼20 nucleotides or base pairs) with Qubit miRNA (microRNA) Assay Kits. These miRNA quantification kits enable quick detection of all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay is highly selective for small RNA over rRNA or large mRNA, and can tolerate contaminants such as salts, solvents, or detergents.

The Qubit miRNA Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of microRNA samples. This assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Depending on sample volume, the assay kit is designed to be accurate for initial miRNA sample concentrations of 0.025 to 150 ng/μL, providing a detection range of 0.5−150 ng.

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit Fluorometer.

Notes
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications

Detection Method Fluorescence
For Use With (Equipment) Qubit Fluorometer
For Use With (Application) microRNA Quantitation
No. of Reactions 500 Reactions
Product Line Qubit
Product Type microRNA Assay Kit
Quantity 500 assays
Shipping Condition Room Temperature
Excitation/Emission 498/518

Frequently Asked Questions (FAQs)

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.
Can the Qubit kits give an indication of sample quality in nucleic acid samples?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.
Can I use the Quant-iT and Qubit Kits with other fluorometers?

All Quant-iT and Qubit kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won't have access to the algorithm in the Qubit fluorometer for generating the standard curve provided by the instrument, instead, you must make a few dilutions of the highest standard DNA or RNA (Standard #2) in the Qubit kits to generate a standard curve with multiple data points.

For Research Use Only. Not for use in diagnostic procedures.