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Invitrogen™ Qubit™ microRNA Assay Kits
Description
Achieve accurate and precise quantification of small RNA (∼20 nucleotides or base pairs) with Qubit miRNA (microRNA) Assay Kits. These miRNA quantification kits enable quick detection of all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay is highly selective for small RNA over rRNA or large mRNA, and can tolerate contaminants such as salts, solvents, or detergents.
The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit Fluorometer.
Notes
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer
Specifications
Specifications
| Detection Method | Fluorescence |
| For Use With (Equipment) | Qubit Fluorometer |
| For Use With (Application) | microRNA Quantitation |
| No. of Reactions | 100 Reactions |
| Product Line | Qubit |
| Product Type | microRNA Assay Kit |
| Quantity | 100 assays |
| Shipping Condition | Room Temperature |
| Excitation/Emission | 498/518 |
Frequently Asked Questions (FAQs)
Yes, the tag does not interfere with the signal.
The exact excitation/emission wavelength information is proprietary. Here are the approximate excitation/emission wavelengths:
- Qubit dsDNA HS Assay: ~500 nm/ ~530 nm
- Qubit dsDNA BR Assay: ~510 nm/ ~530 nm
- Qubit ssDNA Assay: ~490 nm/ ~520 nm
- Qubit RNA HS Assay: ~640 nm/ ~670 nm
- Qubit RNA BR Assay: ~640 nm/ ~670 nm
- Qubit microRNA Assay: ~500 nm/ ~520 nm
- Qubit Protein Assay: ~470 nm/ ~570 nm
The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.
No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.
If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.
For Research Use Only. Not for use in diagnostic procedures.