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QuantStudio™ Dx Real-Time PCR Instrument with 96-well standard block

Catalog No. 4479888
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Catalog No. 4479888 Supplier Thermo Fisher Scientific Supplier No. 4479888

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Specifications

Block Format Interchangeable
Format 96-well Plate
Sensitivity Down to 1 Copy
Reaction Volume Range 10-100 μL
Dynamic Range Up to 9 Logs of Linear Dynamic Range
Content And Storage 96-well Standard Block
Calibrated Dye FAM™, VIC™, ROX™, TAMRA, CY™5
For Use With (Equipment) QuantStudio™ Dx
Thermal Uniformity ±0.5°C
Reaction Speed Standard
Detection Method Fluorescence
Optics 6 Emission Filters (500-720 nm), 6 Excitation Filters (450-670 nm)
Dimensions 53.0 cm (20.9 in.) W x 70.0 cm (27.6 in.) D x 75.0 cm (29.5 in.) H
Capacity 1 Plate
Software Compatibility QuantStudio™ Dx Software; QuantStudio™ Test Development Software
Product Line QuantStudio
Quantity 1 Instrument
Weight 70 kg
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What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

I recently switched the block in my real-time PCR instrument. Now it shows the status as 'not calibrated' for all the calibration plates, even though my calibrations are current. What is causing this issue?

The issue might be due to the block not being properly seated in the instrument. Please follow the steps below:

  1. Remove the block from the instrument.
  2. Reinsert the block into the instrument.


If the problem persists after reseating the block, please contact our Technical Support team for further assistance.

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.