Invitrogen™ Quant-iT™ RNA Assay Kits and Quant-iT RNA HS Reagent 

Description

Enable easy and accurate RNA quantitation with the Quant-iT RNA Assay Kit, which is also available in broad range (BR) and extended range (XR) configurations, as well as a stand-alone high sensitivity (HS) reagent. These Quant-iT RNA quantification kits are highly selective for RNA over dsDNA and can be used to generate fluorescence-based linear detection ranges of RNA.

Quant-iT RNA Assay Kit
The Quant-iT RNA Assay Kit makes RNA quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. The assay is highly selective for RNA over double-stranded DNA, and in the range of 5-100 ng of RNA, the fluorescence signal is linear.

Quant-iT RNA XR (Extended Range) Assay Kit
The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 ng/µL to 10,000 ng/µL depending on sample volume, providing a linear detection range of 200–10,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a fluorescent-based microplate reader.

Quant-iT RNA BR (Broad Range) Assay Kit
The Quant-iT RNA BR (Broad Range) Assay Kit provides an accurate and selective method for quantitation of high-abundance RNA samples. Whereas the original Quant-iT RNA kit has great sensitivity, the Quant-iT RNA Broad Range kit is ideal for microarray samples where the concentration of RNA is higher. With the Quant-iT RNA Broad Range kit, dilution of the RNA samples prior to quantitation is not required. The kit is specific for RNA and will not quantitate DNA, protein, or free nucleotides.

Quant-iT RNA HS Reagent
The Quant-iT RNA HS Reagent makes RNA quantitation easy and accurate, with a linear detection range of 5–100 ng in sample volumes of 1–20 µL and selectivity for RNA, even in the presence of an equal mass of DNA. Using the protocol provided, this concentrated assay reagent provides sufficient material to perform at least 2000 assays in a 96-well microplate format. The assay is performed at room temperature, and the signal is stable for approximately three hours.

Important Features of Quant-iT RNA HS Reagent
• Accurately detects as little as 2 ng RNA in a microplate well
• Core dynamic range of 5–100 ng
• Approximate fluorescence excitation/emission maxima of 644/673 nm

Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in all assay kits.