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Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Catalog No. 12173033
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Quantity:
384 Preps
750 mL
2 x 1 L
5 x 1 L
Product Type:
RNA Lysis Buffer
RNA Purification Kit
RNA Wash Buffer I
RNA Wash Buffer II (5X)
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Catalog No. Quantity Product Type
12173033 2 x 1 L RNA Wash Buffer II (5X)
12173011A 384 Preps RNA Purification Kit
12173022 750 mL RNA Lysis Buffer
12173032 5 x 1 L RNA Wash Buffer I
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Catalog No. 12173033 Supplier Invitrogen™ Supplier No. 12173033
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The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes.

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design

The PureLink Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Specifications

Concentration 5X
Content And Storage Store at room temperature.
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Product Type RNA Wash Buffer II (5X)
Quantity 2 x 1 L
If I do not use all the wells in the filter plate of the PureLink Pro 96 Purification System, can I use those wells at another time?

Yes. Keep the unused wells covered with Foil Tape. Please note that extra Foil Tape and Receiver Plates will be needed.

My centrifuge does not reach 3,000 x g. For the PureLink Pro 96 system, could I spin at a lower force for a longer time?

Generally speaking, yes. It should work the same if you can keep the same g (rcf) x time.

For Research Use Only. Not for use in diagnostic procedures.