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  5. Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Catalog No. 12173011A
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Quantity:
384 Preps
750 mL
2 x 1 L
5 x 1 L
Product Type:
RNA Lysis Buffer
RNA Purification Kit
RNA Wash Buffer I
RNA Wash Buffer II (5X)
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Catalog No. Quantity Product Type
12173011A 384 Preps RNA Purification Kit
12173022 750 mL RNA Lysis Buffer
12173033 2 x 1 L RNA Wash Buffer II (5X)
12173032 5 x 1 L RNA Wash Buffer I
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Catalog No. 12173011A Supplier Invitrogen™ Supplier No. 12173011A
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Description

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes.

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design

The PureLink Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Specifications

Content And Storage • 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
• 250 mL PureLink Pro 96 Wash Buffer I; room temperature
• 250 mL PureLink Pro 96 Wash Buffer II; room temperature
• 75 mL PureLink Pro 96 RNase-free Water; room temperature
• 4 PureLink 96-well Total RNA Filter Plates; room temperature
• 4 PureLink Pro 96 Elution Plates; room temperature
Isolation Technology Silica Plate
Purification Time 25 min. (After sample homogenization)
Elution Volume 45–170 μL
Sample Type Bacteria, Cells, Plant, Tissue, Yeast
Final Product Type Total RNA
High-throughput Compatibility High-throughput Compatible
For Use With (Equipment) Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Product Type RNA Purification Kit
Quantity 384 Preps
Scale Mini
Shipping Condition Room Temperature
Starting Material Amount Bacteria: ≤109 cells, Cells: varies, Plant: ≤150 mg, Tissue: ≤100 mg, Yeast: ≤1.8 mL
Yield Up to 33 μg
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Frequently Asked Questions (FAQs)

If I do not use all the wells in the filter plate of the PureLink Pro 96 Purification System, can I use those wells at another time?

Yes. Keep the unused wells covered with Foil Tape. Please note that extra Foil Tape and Receiver Plates will be needed.
My centrifuge does not reach 3,000 x g. For the PureLink Pro 96 system, could I spin at a lower force for a longer time?

Generally speaking, yes. It should work the same if you can keep the same g (rcf) x time.

For Research Use Only. Not for use in diagnostic procedures.