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Cytiva Protein A HP SpinTrap™ Column

Catalog No. 45001483
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Catalog No. 45001483 Supplier Cytiva Supplier No. 28903132
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For simple, small-scale antibody purification or enrichment of specific proteins by antibody capture in single-use spin columns

Prepacked with Protein A Sepharose™ High Performance Media for coupling of antibodies of IgG subclasses; used with a standard microcentrifuge

  • Fast binding kinetics and high capacity provide high yield
  • Classic (antigen eluted with antibody) and cross-link (antigen eluted separately) protocols provide flexibility
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows
  • Easy scale-up with HiTrap Protein A HP prepacked columns
  • Additional protocol for small-scale purificaiton of monoclonal and polyclonal antibodies from serum and cell culture supernatants

Specifications

Binding Capacity >1 mg Human IgG well
For Use With (Application) Centrifugation
Sample Volume 600 μl
For Use With (Equipment) Standard microcentrifuge.
Content And Storage 2°C to 30°C, 20% Ethanol
Quantity 16 Columns