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Invitrogen™ eBioscience™ Propidium Iodide

Catalog No. 5018262
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1.5 mL
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Catalog No. Quantity
5018262 1.5 mL
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Catalog No. 5018262 Supplier Invitrogen™ Supplier No. BMS500PI

Description

Includes

Propodium Iodide

Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.

  • PI should be analyzed in the PE channel when used as a counterstain for Annexin V FITC
  • 7-AAD can be used in place of PI when using Annexin V PE
  • The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions
  • Choose Propidium Iodide as a counterstain with Annexin V apoptosis assays

Specifications

Color Red
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Propidium Iodide
Dye Type Cell-Impermeant
Emission 535/617
Product Line eBioscience
Quantity 1.5 mL

Frequently Asked Questions (FAQs)

What is the concentration of eBioscience Propidium Iodide?

eBioscience Propidium Iodide is supplied at a concentration of 0.02 mg/mL and we recommend using 10 µL in a total staining volume of 200 µL.

For Research Use Only. Not for use in diagnostic procedures.