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Promega™ TNT™ Quick Coupled Transcription/Translation System

Convenient cell-free system combines RNA polymerase, nucleotides, salts, amino acids, and Recombinant RNasin™ Ribonuclease Inhibitor with reticulocyte lysate solution in a single TNT Quick Master Mix

$202.23 - $739.37

Specifications

For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Storage Requirements Store at -70°C; do not freeze-thaw the lysate more than two times.
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Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

PR-L1170

 
promega™
L1170
8 × 200μL TNT Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 250μL Luciferase Assay Reagent, 50μL Methionine, 1250μL Nuclease-free Water 40 Reactions Each for $739.37

PR-L1171

 
promega™
L1171
200μL TNT T7 Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 50μL Methionine 5 Reactions Each for $202.23

PR-L2080

 
promega™
L2080
8 × 200μL TNT SP6 Quick Master Mix, 5μg Luciferase SP6 Control DNA, 50μL Methionine, 250μL Luciferase Assay Reagent, 1250 Nuclease-free Water 40 Reactions Each for $739.37

PR-L2081

 
promega™
L2081
200μL TNT SP6 Quick Master Mix, 5μg Luciferase SP6 Control DNA, 50μL Methionine 5 Reactions Each for $203.30
Description & Specifications

Specifications

For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Storage Requirements Store at -70°C; do not freeze-thaw the lysate more than two times.

  • Optimized for transcription/translation of genes cloned downstream from either the T7 or SP6 RNA polymerase promoter
  • Produces two- to six-fold more protein in a 60- to 90-minute reaction than do standard in vitro rabbit reticulocyte lysate reactions using RNA templates
  • Includes Luciferase Control DNA; 40-reaction systems also include Luciferase Assay Reagent

U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents.
U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329 and other patents.
U.S. Patent 5,552,302; Australian Patent 646803 and other patents.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
For Laboratory Use. Any use of the product for diagnostics requiring clearance or approval by the FDA may require a license under Mayo Clinic U.S. Patents 6,027,913 and 6,361,949.