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  5. Promega pRL Family of Renilla Luciferase Control Reporter Vectors

Promega pRL Family of Renilla Luciferase Control Reporter Vectors

Catalog No. PRE2241
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Vector:
pRL-CMV vector
pRL-null vector
pRL-SV40 vector
pRL-TK vector
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Catalog No. Vector
PRE2241 pRL-TK vector
PRE2271 pRL-null vector
PRE2261 pRL-CMV vector
PRE2231 pRL-SV40 vector
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Catalog No. PRE2241 Supplier Promega Supplier No. E2241
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Description

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

  • Four Vectors that Contain Wild-Type Renilla Luciferase for Normalization in Reporter Assays
  • A T7 promoter is located immediately upstream of Rluc for in vitro synthesis of Renilla luciferase
  • The SV40 late poly(A) signal sequence is positioned downstream of Rluc to provide efficient transcription termination and mRNA polyadenylation
  • A prokaryotic origin of replication and β-lactamase gene allow selected propagation of the pRL vectors in E. coli host strains

Specifications

Promoter HSV-TK
Reporter Gene Luciferase
Storage Buffer TE Buffer (pH 7.4)
Content And Storage -30°C to -10°C
Quantity 20 μg
Vector pRL-TK vector
For Use With (Application) Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells

Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patent 2,105,984 and related patents.