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Promega™ pGEM™ Vectors

Enable blue/white screening and contain both the SP6 and T7 RNA polymerase promoters

$127.33 - $161.57

Specifications

Promoter Sp6 and T7
For Use With (Application) Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Includes 500μL Glycerol stock of bacterial strain JM109
Quantity 20μg
Storage Requirements Vector at -20°C and bacterial strain at -70°C
View More Specs
Includes:  Vector, 20μg; Bacterial Strain JM109, 500μL
Products
Catalog Number Mfr. No. Restriction Site Vector Price Quantity    

PR-P2241

 
promega™
P2241
ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI pGEM-5Zf(+) vector Each for $161.57

PR-P2251

 
promega™
P2251
ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI pGEM-7Zf(+) vector Each for $161.57

PR-P2151

 
promega™
P2151
Acc I, Ava I, BamH I, EcoRI, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, Xma I pGEM-3Z vector Each for $127.33

PR-P2261

 
promega™
P2261
EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII pGEM-3Zf(-) vector Each for $127.33

PR-P2271

 
promega™
P2271
EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII pGEM-3Zf(+) vector Each for $127.33

PR-P2411

 
promega™
P2411
SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII pGEM-11Zf(+) vector Each for $161.57
Description & Specifications

Specifications

Promoter Sp6 and T7
For Use With (Application) Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Includes 500μL Glycerol stock of bacterial strain JM109
Quantity 20μg
Storage Requirements Vector at -20°C and bacterial strain at -70°C

 

  • Multiple cloning site provides a selection of restriction sites and is flanked by the SP6 and T7 RNA polymerase promoters
  • lacZ α-peptide and multiple cloning site arrangement from pUC18 allow recombinants to be selected using blue/white screening
pGEM-3Z and -4Z Vectors†
  • Versatile vectors can be used for standard cloning and in vitro transcription
  • pGEM-3Z and pGEM-4Z vectors are identical except for the orientation of the SP6 and T7 promoters
pGEM-3Zf(+/-), -5Zf(+/-), -7Zf(+/-), -9Zf(-), -11Zf(+/-), and -13Zf(+) Vectors†
  • Vectors are derived from the pGEM-3Z vector and contain the origin of replication of the filamentous phage f1
  • Versatile vectors can be used for standard cloning, ssDNA production, and in vitro transcription
  • (+) and (-) vectors with the same numeric designation are identical except for the orientation of the f1 origin
  • pGEM-5Zf(+/-) and -7Zf(+/-) contain restriction site arrangements designed to generate unidirectional deletions with the Erase-a-Base™ System
  • pGEM-9Zf(-) permits the excision of an insert containing the SP6 and T7 polymerase promoters