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Promega™ pGEM™-T and pGEM™-T Easy Vector Systems

Convenient systems for the routine subcloning of PCR products

$169.06 - $343.47

Specifications

Promoter Sp6 and T7
For Use With (Application) For the routine subcloning of PCR products
Quantity 20 Reactions
View More Specs
Includes:  Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)
Products
Catalog Number Mfr. No. Includes Restriction Site Storage Requirements Vector Price Quantity    

PR-A1360

 
promega™
A1360
1.2μg pGEM-T easy vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase BstZI, NotI and EcoRI -20°C pGEM-T easy vector I Each for $205.44

PR-A1380

 
promega™
A1380
1.2μg pGEM-T easy vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase, JM109 1.2mL competent cells high efficiency (6 x 200μL) BstZI, NotI and EcoRI Store competent cells at -70°C, store all other components at -20°C pGEM-T easy vector II Each for $343.47

PR-A3610

 
promega™
A3610
1.2μg pGEM-T vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase, JM109 1.2mL competent cells high efficiency (6 x 200μL) BstZI Store competent cells at -70°C, store all other components at -20°C pGEM-T vector II Each for $309.23

PR-A3600

 
promega™
A3600
1.2μg pGEM-T vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase BstZI -20°C pGEM-T vector I Each for $169.06
Description & Specifications

Specifications

Promoter Sp6 and T7
For Use With (Application) For the routine subcloning of PCR products
Quantity 20 Reactions

The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.

  • 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
  • f1 Origin of Replication allows the preparation of single-stranded DNA

pGEM-T Vector Systems

  • Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert
  • Double digestion may also be used to release the insert from the vector
pGEM-T Easy Vector Systems
  • Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion