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Promega pGEM™-T and pGEM™-T Easy Vector Systems 
Convenient systems for the routine subcloning of PCR products
$247.71 - $499.37
Specifications
| Promoter | SP6, T7 |
|---|---|
| Quantity | 20 Reactions |
| For Use With (Application) | For the routine subcloning of PCR products |
Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)
| Catalog Number | Mfr. No. | Vector | Restriction Site | Price | Quantity | ||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Vector | Restriction Site | Price | Quantity | ||||
|
PR-A1360
|
Promega
A1360 |
pGEM-T easy vector I | BstZI, NotI and EcoRI |
Each for $299.09
|
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|
PR-A1380
|
Promega
A1380 |
pGEM-T easy vector II | BstZI, NotI and EcoRI |
Each for $499.37
|
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|
PR-A3610
|
Promega
A3610 |
pGEM-T vector II | BstZI |
Each for $449.31
|
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PR-A3600
|
Promega
A3600 |
pGEM-T vector I | BstZI |
Each for $247.71
|
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Description
The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.
- 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
- f1 Origin of Replication allows the preparation of single-stranded DNA
pGEM-T Vector Systems
- Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert Double digestion may also be used to release the insert from the vector
- Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion
Specifications
| SP6, T7 | |
| 20 Reactions | |
| For the routine subcloning of PCR products |