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Promega M-MLV Reverse Transcriptase
M-MLV RT is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).
Manufacturer: Promega M1705
Description
- RNA-Dependent DNA Polymerase for cDNA Synthesis
- Enzyme supplied at a concentration of 200u/µL
Specifications
200U/μL | |
Liquid | |
Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50% | |
7.5 | |
M-MLV Reverse Transcriptase |
First-strand cDNA synthesis, primer extension | |
Recombinant E. coli Strain | |
−30 to −10°C | |
5X reaction buffer (250mM Tris-HCl [pH 8.3 at 25°C], 375mM KCl, 15mM MgCl2, 50mM DTT), buffer also available separately | |
50000U |
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