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Promega CheckMate™ Mammalian Two-Hybrid System DFS Item

Powerful system for detecting protein:protein interactions in vivo.

Supplier:  Promega E2440

Catalog No. PR-E2440


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Includes:

pACT vector, 20μg; pBIND vector, 20μg; pBIND-Id control vector, 20μg; pACT-MyoD control vector, 20μg; pG5luc vector, 2 x 20μg


Description

Description

  • Powerful System for Detecting Protein:Protein Interactions In Vivo
  • Mammalian system for studying interactions in cell line of choice
  • Study post-translational modifications including glycosylation, phosphorylation and acylation

Order Info

The Dual-Luciferase Reporter Assay System (sold separately) is used for detection

  • U.S. Patent 5,670,356.
  • Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patent 2,105,984 and related patents.
  • The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

Specifications

Specifications

Confirming suspected interactions between two Proteins and identifying domains/residues involved in Protein-Protein interactions
−30 to −10°C
Each
20μg pACT vecto r, 20μg pBIND vecto r, 20μg pBIND Id control vecto r, 20μg pACT MyoD control vecto r, 2 x 20μg pG5luc vecto r
Hybrid system
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