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Applied Biosystems™ ProFlex™ PCR System + Plastics Package, 2x384-well, EnduraPlates

Catalog No. A41187
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Catalog No. A41187 Supplier Applied Biosystems™ Supplier No. A41187

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Applied Biosystems™ ProFlex™ PCR System + Plastics Package with EnduraPlates combines reliability and performance with flexible configuration and control features that adapts to your research needs.

  • Interchange the 96-well 0.2 mL block with another block type if your throughput needs change
  • Access the system remotely (and conveniently) through a mobile app
  • Program the instrument in seconds with a simple-to-use touchscreen interface
  • Simulate your old instrument with Thermal Simulation modes
  • Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks
  • Optimized for performance with Applied Biosystems instruments
  • Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
  • Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
  • Excellent mechanical stability and flatness for your complete workflow needs

Specifications

Thermal Accuracy ±0.25°C (35°C to 99.9°C)
Content And Storage • ProFlex 2 x 384-well PCR System
• 5 x MicroAmp EnduraPlate Optical 384-well Clear Reaction Plates with Barcode (100 plates total)
• 1 x MicroAmp Clear Adhesive Films (100 films)
High-throughput Compatibility High-throughput Compatible
Thermal Uniformity <0.5°C (20 sec. after reaching 95°C)
Reaction Speed Fast, Standard
Dimensions 22 x 13 x 10.6 in. (56.5 x 33 x 27.2 cm)
Capacity 768 x 20 μL tubes, 2 x 384-well plate
Voltage 100/240 V
Display Type 8.4 in. Color TFT LCD Touchscreen
Product Type PCR System
Warranty Limited Warranty
Thermal Range 0°C to 100°C
User Interface USB, WiFi, Cloud, Ethernet
Max. Ramp Rate 3°C/sec. (Block), 1.6°C/sec. (Sample)
Quantity 1 Package
Weight 45 lb. (20.4 kg)
Block Configurations 2 x 384-well Isothermal Blocks
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Frequently Asked Questions (FAQs)

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

For Research Use Only. Not for use in diagnostic procedures.