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Applied Biosystems™ ProFlex™ PCR System, 96-well
Description
The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you've come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
The ProFlex 96-well PCR system allows you to:
- Interchange the 96-well 0.2-mL block with another block type if throughput needs change
- Access the system remotely (and conveniently) through a mobile app
- Program the instrument in seconds with a simple-to-use touchscreen interface
- Simulate an old instrument with Thermal Simulation modes
- Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks
Interchangeable blocks
If your research requirements change in the future, or if you share the system with someone who requires a different format, simply remove the 96-well block from the top of the instrument and exchange it with another block type. This step takes typically less than 15 seconds and requires no tools or kits.
Mobile app connectivity
Have you ever set up all of your PCR reactions, only to find out that the thermal cycler is already in use? Check its availability with a mobile app before you get to the lab. Also download experimental report files, receive alerts, and more!
Simulation modes
The ProFlex PCR System is equipped with simulation modes that mimic your old thermal cycler's ramp rate. Simulation modes are available for MJ Research PTC 200, Bio-Rad™ C1000, Bio-Rad MyCycler™, Eppendorf™ Mastercycler™, Applied Biosystems 9700, Applied Biosystems 9600, and Applied Biosystems Veriti systems.
VeriFlex blocks: enhanced PCR functionality
VeriFlex blocks are controlled by multiple Peltier elements (six separate Peltier elements per 96-well module). This gives you the ultimate control of your block for precise, 'better-than-gradient' optimization experiments and for running multiple reaction conditions at once for precise incubations and other non-PCR workflows.
8.4-inch touchscreen control
The simple and intuitive touchscreen interface allows you to program with ease and limits the amount of time spent training yourself and others how to use the system.
Specifications
Specifications
| Block Format | Interchangeable |
| Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
| High-throughput Compatibility | High-throughput Compatible |
| Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
| Reaction Speed | Fast, Standard |
| Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
| Capacity | 96-well, 0.2 mL Tubes |
| Voltage | 100/240 V |
| Product Line | ProFlex |
| Display Type | 8.4 in. Color TFT LCD Touchscreen |
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Frequently Asked Questions (FAQs)
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
For Research Use Only. Not for use in diagnostic procedures.