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  5. Invitrogen™ Human Procalcitonin ELISA Kit

Invitrogen™ Human Procalcitonin ELISA Kit

Catalog No. EHPCT
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96 Tests
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Catalog No. Quantity
EHPCT 96 Tests
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Description

ELISA

The Human Procalcitonin ELISA quantitates Hu Procalcitonin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Procalcitonin. Principle of the method The Human Procalcitonin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

Accession Number P01258, P06881
Assay Range 30-20000 pg/mL
Assay Sensitivity 30 pg/mL
Calibration Range 30 pg/mL to 20000 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family GPCR (G-Protein Coupled Receptors)
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Substrate Type Adaptor Proteins
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias alpha-type CGRP; CALC1; CALCA; Calcitonin; calcitonin 1; Calcitonin carboxyl-terminal peptide; Calcitonin gene-related peptide 1; Calcitonin gene-related peptide I; calcitonin related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; calcitonin; calcitonin gene-related peptide 1; calcitonin-related polypeptide alpha; CCP; CGRP; CGRP1; CGRP-I; CT; Katacalcin; KC; PCT; PDN-21alpha-type CGRP; CALC1; CALCA; Calcitonin; calcitonin 1; Calcitonin carboxyl-terminal peptide; Calcitonin gene-related peptide 1; Calcitonin gene-related peptide I; calcitonin related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; calcitonin; calcitonin gene-related peptide 1; calcitonin-related polypeptide alpha; CCP; CGRP; CGRP1; CGRP-I; CT; Katacalcin; KC; PCT; PDN-21; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT
Gene ID (Entrez) 796
Gene Symbol CALCA
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases Calcitonin carboxyl-terminal peptide, CCP, PDN-21
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Neurobiology
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym CALC1, CGRP, CGRP-I, CGRP1, CT, KC
Target Procalcitonin
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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