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Invitrogen™ Pregnenolone Competitive ELISA Kit

Catalog No. EEL162
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Quantity:
96 Tests
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Catalog No. Quantity
EEL162 96 Tests
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Catalog No. EEL162 Supplier Invitrogen™ Supplier No. EEL162
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Description

ELISA

Pregnenolone is a steroid hormone that serves as a precursor for other hormones. It is primarily produced in the adrenal glands, gonads, and brain. Pregnenolone plays a role in steroid hormone synthesis and acts as a neurosteroid, potentially affecting brain function. Structurally, it is derived from cholesterol and consists of a four-ring structure.

Specifications

Assay Range 0.78 to 50 ng/mL
Assay Sensitivity 0.47 ng/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Pregnenolone
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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