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Gibco™ pRABBIT IgG IRES-EmGFP Positive Control Vector

Catalog No. A39243
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A39243 1 Vial
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Catalog No. A39243 Supplier Gibco™ Supplier No. A39243
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Description

pRABBIT IgG IRES-EmGFP Positive Control Vector is a mammalian expression control that expresses a complete, full-length rabbit IgG and EmGFP.

Can be used as a positive expression control for transient expression systems such as the Expi293 Expression System, the ExpiCHO Expression System, the FreeStyle 293 Expression System, and the FreeStyle CHO Expression System.

Features:

  • A mix of pcDNA3.4 plasmids containing the coding sequences for EmGFP and the heavy and light chains of rabbit IgG
  • Optimized mix of IgG light and heavy chain transfection-grade plasmids in a single, ready-to-use tube
  • Expression of rabbit IgG at 450 to 500 mg/L in Expi293F cells
  • Sufficient material for transfection of up to 150 mL of suspension culture in the Expi293, ExpiCHO, and FreeStyle systems

This control is included in the Expi293 GnTI-, Expi293 Inducible and Expi293 Inducible GnTi- expression system kits.

Specifications

Product Type Positive Control DNA Vector
Content And Storage 1 vial (1 mg/mL) pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C
Shipping Condition Dry Ice
Quantity 1 Vial
Grade Transfection Grade

Frequently Asked Questions (FAQs)

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.

For Research Use Only. Not for use in diagnostic procedures.