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  5. Invitrogen™ Mouse PLGF-2 (PGF) ELISA Kit

Invitrogen™ Mouse PLGF-2 (PGF) ELISA Kit

Catalog No. EMPGF
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96 Tests
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Catalog No. Quantity
EMPGF 96 Tests
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Description

ELISA

The Mouse PLGF-2 (PGF) ELISA quantitates Ms PLGF-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PLGF-2. Principle of the method The Mouse PLGF-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor affects different cell types and regulates various biological responses. The activity of vascular endothelial growth factor (VEGF), which is the most potent of all angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in human placenta.

Specifications

Accession Number P49764
Assay Range 1.64 to 400 pg/mL
Assay Sensitivity 1 pg/mL
Calibration Range 1 pg/mL to 400 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Growth Factors & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity The sandwich ELISA antibody pair detects Mouse PLGF-2.
Sufficient For 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 18654
Gene Symbol Pgf
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Development
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym AI854365, PIGF, Plgf
Target PGF
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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