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Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer

Catalog No. pi37573
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Quantity:
100 mL
1 L
Chemical Name or Material:
Protein-Free (PBS)
Protein-Free (TBS)
Protein-Free T20 (PBS)
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Catalog No. Quantity Chemical Name or Material
PI37573 1 L Protein-Free T20 (PBS)
PI37584 100 mL Protein-Free (PBS)
PI37572 1 L Protein-Free (PBS)
PI37585 100 mL Protein-Free (TBS)
PI37570 1 L Protein-Free (TBS)
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Catalog No. PI37573 Supplier Thermo Scientific™ Supplier No. 37573
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Description

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Features of Protein-Free Blocking Buffer include:

  • Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
  • Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
  • Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
  • High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Specifications

Chemical Name or Material Protein-Free T20 (PBS)
Concentration 1X
For Use With (Application) ELISA
Physical Form Liquid
Product Line Pierce
Quantity 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.
Is the Pierce Protein-Free Blocking Buffer (Cat. No. 37571) animal-origin free (AOF)?

Yes, it is animal-origin free (AOF).