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Thermo Scientific™ Pierce™ Protein A/G UltraLink™ Resin

Extremely durable polyacrylamide support with immobilized recombinant Protein A/G for various IgG purification methods.

$514.50 - $1,711.50

Specifications

Description Pierce Protein A/G UltraLink Resin
Format Resin slurry
Formulation Protein A/G on UltraLink Resin, slurried in water with sodium azide
Sufficient For Binding 20mg human IgG/mL resin
Support Type Acrylamide
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

PI53132

 
thermo scientific™
53132
2mL Pack for $514.50

PI53133

 
thermo scientific™
PI53133
10mL Pack for $1,711.50
Description & Specifications

Specifications

Description Pierce Protein A/G UltraLink Resin
Format Resin slurry
Formulation Protein A/G on UltraLink Resin, slurried in water with sodium azide
Sufficient For Binding 20mg human IgG/mL resin
Support Type Acrylamide
Target IgG

Thermo Scientific™ Pierce Protein A/G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein A/G has been immobilized for use in IgG purification methods.

The Protein A/G UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A/G is a recombinant fusion protein consisting of the antibody-binding domains of Protein A and Protein G, enabling antibody purification from serum samples of a variety of species.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

  • Immobilized Protein A/G – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG

  • Standard capacity – loading of immobilized Protein A/G provides a binding capacity of approx. 20mg human IgG per milliliter of resin

  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)