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Thermo Scientific™ Pierce™ Protein A/G Plus Agarose

Catalog No. PI20424
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2 mL
20 mL
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Catalog No. Quantity
PI20424 20 mL
PI20423 2 mL
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Catalog No. PI20424 Supplier Thermo Scientific™ Supplier No. 20424
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Description

  • Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables polyclonal IgG purification from nearly any mammalian species
  • Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
  • Inert and stable – superior manufacturing method immobilizes Protein A/G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
  • High capacity – this 'Plus' variety of Pierce Protein A/G Agarose has a very dense load of immobilized Protein A/G, providing a binding capacity greater than 50 mg human IgG/mL resin

Specifications

Product Type Agarose
Stationary Phase Protein A/G
Purification Target IgG Antibodies, Ig Antibodies
For Use With (Application) Antibody Purification
Content And Storage Store in refrigerator (2–8°C).
Kit Contents Protein A/G, Agarose Resin
Quantity 20 mL
Shipping Condition Wet Ice
Form Agarose Bead

Frequently Asked Questions (FAQs)

I have noticed that there is a slightly higher amount of agarose resin in product Cat. No. 20424 than expected according to the manual. What is the reason for that?

To ensure the delivery of the correct amount of agarose resin, the products are slightly overfilled with resin. The slurry will be maintained at 50% so the resin and liquid solution volume will be equivalent.

What does it mean that the crosslinked 6% beaded agarose is supplied as a 50% slurry for products Pierce Protein A/G Plus Agarose (Cat. No. 20423 and 20424) and Pierce Protein A/G Agarose (Cat. No. 20421 and 20422)?

This means that the solution consists of 50% settled resin and 50% liquid solution. For example, Cat. No. 20424 contains 20 ml of settled resin and 20 ml of storage buffer, making it a 40 ml, 50% slurry.

For Research Use Only. Not for use in diagnostic procedures.