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Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase

Catalog No. PI31487
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1 mg
5 x 1mg
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PI31487 1 mg
PI31488 5 x 1mg
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Catalog No. PI31487 Supplier Thermo Scientific™ Supplier No. 31487
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Description

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates.

Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate. Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines. Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody. After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine. The conjugate is purified by desalting or by dialysis. If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No. 44920) to separate unreacted enzyme from the conjugate.

Highlights:

  • Reacts with readily accessible primary amines to form a covalent secondary amine bond
  • Can be stored for at least 12 months at -20°C
  • Consistent conjugation yields reliable conjugates
  • Enzyme activity is 120-200 units/mg

Specifications

Chemical Reactivity Amine
Quantity 1 mg
Solubility Water
Reactive Moiety Aldehyde
Content And Storage Note: EZ-Link Plus Activated Peroxidase contains 1-3 moles of aldehyde per mole of peroxidase.

Storage: Upon receipt store product protected from moisture at -20°C. Product is shipped with an ice pack.
Form Solid
Product Line EZ-Link
Label Type Enzyme Labeled
Label or Dye HRP (Horseradish Peroxidase)

Frequently Asked Questions (FAQs)

What would be the best way to crosslink an antibody with horseradish peroxidase (‘HRP’ unmodified)?

A commonly used method for conjugating HRP to antibodies is by activation of the HRP using sodium meta-periodate (Cat no. 20504) to generate aldehydes. After the reaction, the HRP must be dialyzed to remove the meta-periodate; then the aldehydes on the HRP will react directly to the amine groups on the antibody. Alternatively, we offer activated HRP (Cat. No. 31488) as an alternative to avoid the periodate oxidation step to modify the HRP. 

For Research Use Only. Not for use in diagnostic procedures.