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Thermo Scientific™ Metal Enhanced DAB Substrate Kit

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Supplier:  Thermo Scientific™ 34065

Catalog No. PI34065


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Description

Description

Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.

Highlights:

  • Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

  • Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

  • Six-hour stability – working solution is stable for more than six hours at room temperature

Includes:

Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL

Specifications

Specifications

Chromogenic, Colorimetric
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)
HRP (Horseradish Peroxidase) Substrate
Immunodetection, IHC (Immunohistochemistry)
275 mL of 1X working solution
DAB (Enhanced)
Pierce
DAB Substrate Kit
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For Research Use Only. Not for use in diagnostic procedures.