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Thermo Scientific™ Metal Enhanced DAB Substrate Kit

Catalog No. PI34065
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275 mL of 1X working solution
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Catalog No. Quantity
PI34065 275 mL of 1X working solution
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Catalog No. PI34065 Supplier Thermo Scientific™ Supplier No. 34065
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Description

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.

Highlights:

  • Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

  • Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

  • Six-hour stability – working solution is stable for more than six hours at room temperature

Includes:

Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL

Specifications

Detection Method Chromogenic, Colorimetric
Quantity 275 mL of 1X working solution
Content And Storage
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)
Substrate DAB (Enhanced)
Substrate Type HRP (Horseradish Peroxidase) Substrate
Product Line Pierce
For Use With (Application) Immunodetection, IHC (Immunohistochemistry)
Product Type DAB Substrate Kit

For Research Use Only. Not for use in diagnostic procedures.