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Thermo Scientific™ Mammalian beta-Galactosidase Assay Kit

Catalog No. PI75707
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75 mL
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PI75707 75 mL
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Catalog No. PI75707 Supplier Thermo Scientific™ Supplier No. 75707

Description

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Highlights:

  • Choose stand-alone assay reagent or complete kit for cell lysis and detection
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatiible with prepared lysates from other species such as bacteria

Includes:

Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution

Specifications

Content And Storage Sufficient For: 250 microplate wells of adherent cells
  • Pierce beta-Galactosidase Assay Reagent, 25 mL
  • M-PER Mammalian Protein Extraction Reagent, 25 mL
  • Stop Solution, 25 mL

    Store the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
    		•
    Description Pierce Mammalian beta-Galactosidase Assay Kit
    Detection Method Absorbance
    For Use With (Application) Absorbance
    For Use With (Equipment) Microplate Reader
    Product Type Beta-Galactosidase Assay Kit
    Dye Type Enzyme Labeled
    Format 96-well Plate
    Product Line Pierce
    Quantity 75 mL
    Substrate Proprietary
    Substrate Type Beta-Gal Substrate
    Sufficient For 250 microplate wells of adherent cells
    Target Beta-Galactosidase
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    Frequently Asked Questions (FAQs)

    I tested the Mammalian beta-Galactosidase Assay Kit and did not use the Stop Solution. I performed three absorbance readings and the values were different, why is this?

    Using the Assay Stop Solution is optional. However, if the Stop Solution is not used, the absorbance values from the plate will change over time. This means that the absorbance needs to be read immediately after the 30 min incubation.
    How do I prepare cells for the Mammalian beta-Galactosidase Assay Kit?

    Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Cat. No.78503) may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate can be used to assay for beta-galactosidase activity. 

    For Research Use Only. Not for use in diagnostic procedures.