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Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Manufacturer:  Thermo Scientific™ PI31494

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Catalog No. PI31494


 

Specifications

Description

Thermo Scientific™ Pierce Maleimide-Activated HRP Conjugation Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues
Description & Specifications

Specifications

1 enzyme-labeling reaction with up to 5mg of antibody (IgG)
Multi-component kit with activated HRP
Maleimide Activated Hrp 1/pk
Maleimide Activated Horseradish Peroxidase, 5mg;;Conjugation Buffer, 20mL;;2-Mercaptoethylamine·HCl, 6mg;;SATA, 2mg;;Dimethylformamide, 1mL;;Hydroxylamine·HCl, 5mg;;Polyacrylamide Desalting Column, 1 x 10mL
1 rxn kit