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Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit


Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Manufacturer: thermo scientific™  PI31494

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Catalog No. PI31494

  • / Pack
 

Description & Specifications

Specifications

Includes Maleimide Activated Horseradish Peroxidase, 5mg;;Conjugation Buffer, 20mL;;2-Mercaptoethylamine·HCl, 6mg;;SATA, 2mg;;Dimethylformamide, 1mL;;Hydroxylamine·HCl, 5mg;;Polyacrylamide Desalting Column, 1 x 10mL
Quantity 1 rxn kit
Item Description Maleimide Activated Hrp 1/pk
Formulation Multi-component kit with activated HRP
Sufficient For 1 enzyme-labeling reaction with up to 5mg of antibody (IgG)

Thermo Scientific™ Pierce Maleimide-Activated HRP Conjugation Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues