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Thermo Scientific™ Pierce™ IP Lysis Buffer

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

$130.20 - $130.20

Specifications

Description Pierce IP Lysis Buffer
Format Liquid
Formulation 25mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% NP-40 and 5% glycerol
Source Material Cultured mammalian cells
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

PI87787

 
thermo scientific™
87787
100mL Each for $130.20
Description & Specifications

Specifications

Description Pierce IP Lysis Buffer
Format Liquid
Formulation 25mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% NP-40 and 5% glycerol
Source Material Cultured mammalian cells

Thermo Scientific™ Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation.


Additional Information and Online Ordering »

The IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pulldown and immunoprecipitation assays.

Highlights:

  • Optimized for compatibility with immunoprecipitation and pull-down assays
  • Compatible with protein assays, reporter assays and immunoassay procedures
  • Ready-made formula is effective for extracting cytoplasmic, membrane and nuclear proteins
  • Gentle formulation helps maintain protein complexes for co-immunoprecipitation (co-IP)
  • Does not liberate genomic DNA which can cause high sample viscosity