missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Extraction
  5. Thermo Scientific™ Pierce™ IP Lysis Buffer

Thermo Scientific™ Pierce™ IP Lysis Buffer

Catalog No. PI87787
Change view
Click to view available options
Quantity:
100 mL
250 mL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI87787 100 mL
PI87788 250 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. PI87787 Supplier Thermo Scientific™ Supplier No. 87787
Only null left

Description

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

Thermo Scientific Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate.

Features of IP Lysis Buffer:

  • Optimized for compatibility with immunoprecipitation and pull-down assays
  • Compatible with protein assays, reporter assays and immunoassay procedures
  • Ready-made formula is effective for extracting cytoplasmic, membrane and nuclear proteins
  • Gentle formulation helps maintain protein complexes for co-immunoprecipitation
  • Does not liberate genomic DNA which can cause high sample viscosity

IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pull-down and immunoprecipitation assays.

Pierce IP Lysis Buffer is effective for lysing cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. Optimized for pull-down and immunoprecipitation assays, this lysis buffer is also compatible with many other applications, including the Thermo Scientific Pierce BCA and 660 nm Protein Assays, protein purification and immunoassays (e.g., ELISA, Western blot).

Pierce IP Lysis Buffer is composed of 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol. The buffer does not contain protease or phosphatase inhibitors; however, if desired, inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail or Phosphatase Inhibitor Cocktail can be added just before use to prevent proteolysis and maintain phosphorylation of proteins.

Related Products

  • M-PER™ Mammalian Protein Extraction Reagent (Cat. No. 78501)
  • RIPA Lysis and Extraction Buffer (Cat. No. 89900)

Specifications

Quantity 100 mL
Format Liquid
Product Type IP Lysis Buffer
Volume (Metric) 100 mL
Content And Storage Upon receipt store at 4°C.
Product Line Pierce

Frequently Asked Questions (FAQs)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).
Can you provide the shelf-life for the Pierce IP Lysis Buffer?

The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Can you provide the shelf life for Pierce IP Lysis Buffer?

Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
Can I store Pierce IP Lysis Buffer (Cat. No. 87787) at room temperature?

Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.
Which protein assays are compatible with the Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.

What is the basic protocol to use with the Thermo Scientific IP Lysis Buffer?

For suspension cells, use 500 µl of lysis buffer per 50 mg of wet cell pellet (10:1 v/w). Incubate lysate on ice for 5 minutes with periodic mixing. Remove cell debris by centrifugation at ~13,000 × g for 10 minutes at 4 degrees C. Transfer supernatant to a new tube.
Does the Thermo Scientific IP Lysis Buffer contain protease inhibitors?

The Thermo Scientific IP Lysis Buffer does not contain protease or phosphatase inhibitors. We recommend inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail (Cat. No. 87786) or Phosphatase Inhibitor Cocktail (Cat. No. 78420) to be added to the buffer just before use.
Why is the Thermo Scientific IP Lysis Buffer the recommended lysis buffer for preparing samples for co-immunoprecipitation (Co-IP)?

The gentle formulation of the buffer helps maintain protein complexes for co-immunoprecipitation.
When should I use Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is recommended for scientists working with mammalian cells in order to extract cellular proteins for Immunoprecipitation and Western Blot analysis. It is effective for lysing cultured mammalian cells from both plated cells and cells pelleted from suspension cultures, and is especially formulated for pull-down and immunoprecipitation assays, and as a wash buffer for affinity resins. This lysis buffer is also compatible with protein purification, immunoassays and ELISA.
What is the composition of the Thermo Scientific IP Lysis Buffer (Cat. No. 87787)?

The Thermo Scientific IP Lysis Buffer contains 25 mM Tris.HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol. This formulation is optimized for compatibility with immunoprecipitation and pull-down assays and does not contain SDS.
Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.
What lysis buffers do you offer for mammalian cells?

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

For Research Use Only. Not for use in diagnostic procedures.