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Thermo Scientific™ Pierce™ IgM Purification Kit

Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Manufacturer: thermo scientific™  PI44897

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Catalog No. PI44897


Description & Specifications


Description Pierce IgM Purification Kit
Formulation Multi-component kit
Quantity 1 column kit
Sufficient For 10 IgM purifications, each from 0.5mL mouse ascites samples
Kit Contents Column Extender
Format Drip column
Support Type Agarose
Target IgM (mouse)

Thermo Scientific™ Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.


  • Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

  • Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use

  • Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

  • Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity


Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender