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Thermo Scientific™ Pierce™ IgM Purification Kit


Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Manufacturer: thermo scientific™  PI22212

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Catalog No. PI22212

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Description & Specifications

Specifications

Description Pierce Mannan Binding Protein Agarose
Formulation Mannan binding protein (MBP) on 4% beaded agarose, slurried in IgM Binding Buffer
Quantity 10mL
Sufficient For Binding 0.3mg mouse IgM from 0.1mL ascites fluid per 1mL resin per use
Format Resin slurry
Support Type Agarose
Target IgM (mouse)

Thermo Scientific™ Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.

Highlights:

  • Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

  • Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use

  • Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

  • Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity

Includes:

Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender