Thermo Scientific™ Pierce™ IgM Purification Kit
Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.
Manufacturer: Thermo Scientific™ PI22212
Thermo Scientific™ Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.
Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.
Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum
Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use
Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies
Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity
Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender
|Pierce Mannan Binding Protein Agarose|
|Mannan binding protein (MBP) on 4% beaded agarose, slurried in IgM Binding Buffer|
|Binding 0.3mg mouse IgM from 0.1mL ascites fluid per 1mL resin per use|