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Thermo Scientific™ Pierce™ Horseradish Peroxidase

Catalog No. PI31490
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Quantity:
10 mg
100 mg
Catalog No. Quantity
PI31490 10 mg
PI31491 100 mg
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Catalog No. PI31490

Supplier: Thermo Scientific™ 31490

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Description

Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

Thermo Scientific™ Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blotting and immunohistochemistry applications.

Highlights:

  • Superior to alkaline phosphatase and β-galactosidase conjugates due to the higher specific enzyme activity
  • Small size (40kDa) allows excellent cellular penetration
  • Variety of substrates available
  • Ideal in blotting and cytochemistry applications
  • Used as the reporter enzyme for Thermo Scientific SuperSignal Chemiluminescent Western Blotting and ELISA Substrates

Note:

Porstmann, B., Porstmann, T., Nugel, E. and Evers, U. (1985). Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase, β-galactosidase, J. Immunol. Meth. 79, 27-37.

Wordinger, R.J., Miller, G.W. and Nicodemus, D.S. (1987). Manual of Immunoperoxidase Techniques, 2nd Edition. Chicago: American Society of Clinical Pathologists Press, pp. 23-24.

Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4(1), 35-68.

Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32, 219-229.

Passey, R.B., et al. (1977). Evaluation and comparison of 10 glucose methods and the reference method recommended in the proposed product class standard. Clin. Chem. 23(1), 131.

Hosoda, H., Takasaki, W., Tsukamoto, R. and Nambara, T. (1987). Sensitivity of steroid immunoassays. Comparison of alkaline phosphatase, β-galactosidase and horseradish peroxidase as labels in a colorimetric assay system. Chem. Pharm. Bull. 35, 3336-3342.

Samoszuk, M.K., et al. (1989). Antibody, Immunoconjugates and Radiopharmaceuticals 2, 37-46.

Specifications

Quantity 10 mg
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Form Powder
For Use With (Application) Western Blotting, ELISA, Immunohistochemistry
Product Line Pierce
Label or Dye HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.