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Thermo Scientific™ Pierce™ High-Capacity Protein A Agarose and Kits

Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits.

$183.75 - $2,572.50

Specifications

Target IgG
View More Specs
Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI22810

 
thermo scientific™
22810
Pierce Protein A Plus Agarose 1mL Each for $183.75

PI22811

 
thermo scientific™
22811
Pierce Protein A Plus Agarose 5mL Pack for $726.60

PI22812

 
thermo scientific™
PI22812
Pierce Protein A Plus Agarose 25mL Pack for $2,572.50

PI45202

 
thermo scientific™
45202
Pierce Protein A Spin Plate for IgG Screening 1 plate Pack for $352.80

PI89924

 
thermo scientific™
89924
Pierce Protein A Chromatography Cartridges, 1mL 2 cartridges Pack of 2 Each for $262.50

PI89925

 
thermo scientific™
89925
Pierce Protein A Chromatography Cartridges, 5mL 1 cartridge Each for $521.85

PI89948

 
thermo scientific™
89948**
NAb Protein A Plus Spin Purification Kit, 0.2mL 10 column kit Each for $402.15

PI89952

 
thermo scientific™
89952
NAb Protein A Plus Spin Columns, 0.2mL 10 columns Pack of 10 Each for $425.25

PI89956

 
thermo scientific™
89956
NAb Protein A Plus Spin Columns, 1mL 5 columns Pack of 5 Each for $688.80

PI89960

 
thermo scientific™
89960
NAb Protein A Plus Spin Columns, 5mL 1 column Each for $756.00

PI89978

 
thermo scientific™
89978
NAb Protein A Plus Spin Purification Kit, 1mL 2 column kit Each for $362.25
Description & Specifications

Specifications

Target IgG

Thermo Scientific™ Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits.

Pierce Protein A Plus Agarose consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available forms of immobilized Protein A affinity resin, this premiere variety provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems. Accordingly, Pierce Protein A Plus Agarose is offered in several convenient package formats, including bottled resin slurries, three sizes of spin columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates.

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Highlights:

  • Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
  • Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
  • Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
  • High capacity – “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34mg human IgG/mL resin (approx. 16 to 17mg mouse IgG/mL resin)
  • Multiple formats – choose from bottled resin slurries, centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates

Properties of crosslinked 6% beaded agarose (CL-6B):

  • Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
  • Average Particle Size: 45 to 165 microns
  • Exclusion Limit: 10,000 to 4,000,000 daltons
  • Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
  • Maximum Linear Velocity: 30cm per hour
  • Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Available Formats:

  • Resin Slurries: 1mL, 5mL, 25mL bottles
  • Spin Columns: 0.2mL (microcentrifuge), 1mL, 5mL columns
  • Purification Kits: complete kits in all three column sizes
  • Chromatography Cartridges - compatible with automated liquid chromatography systems and with syringe processing
  • 96-well Spin Plates: pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
  • Bulk and Custom: let us know your needs for bulk Pierce Protein A Plus Agarose

Includes:

Purification kits contain Protein A Plus Spin Columns, binding buffer, elution buffer and neutralization buffer