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Thermo Scientific™ Pierce™ GST Agarose

Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.

Manufacturer:  Thermo Scientific™ PI20211

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Catalog No. PI20211


 

Specifications

Description

Perform isolation or removal of cross-reacting GST-specific antibodies from serum or other antibody solutions with Thermo Scientific™ Pierce GST Agarose.

This GST resin consists of purified glutatione S-transferase protein from Schistosoma japonicum that has been immobilized on crosslinked 6% beaded agarose. Antibody molecules in that cross-react with the GST portion of a recombinant fusion protein can be removed from the desired protein-specific antibody by passing antiserum over a GST agarose column. GST antibodies will bind to the immobilized GST, whereas antibodies against other fusion proteins will pass through the column unrestrained.

Highlights:

  • Specific – provides purification of target antibodies from those directed against the GST tag

  • Reusable – bound antibodies can be eluted with 6M guanidinium chloride allowing for 10 purifications with each column or aliquot of resin

  • Two formats – choose either pre-packed columns or resin slurry for use at a variety of scales

Description & Specifications

Specifications

Binding approx. 0.5mg anti-GST antibody per mL of resin
Agarose
5mL
Glutathione S-transferase on crosslinked 6% beaded agarose, slurried in water with sodium azide
Pierce GST Agarose