Thermo Scientific™ Pierce™ GST Agarose
Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.
Manufacturer: Thermo Scientific™ PI20211
Perform isolation or removal of cross-reacting GST-specific antibodies from serum or other antibody solutions with Thermo Scientific™ Pierce GST Agarose.This GST resin consists of purified glutatione S-transferase protein from Schistosoma japonicum that has been immobilized on crosslinked 6% beaded agarose. Antibody molecules in that cross-react with the GST portion of a recombinant fusion protein can be removed from the desired protein-specific antibody by passing antiserum over a GST agarose column. GST antibodies will bind to the immobilized GST, whereas antibodies against other fusion proteins will pass through the column unrestrained.
Specific – provides purification of target antibodies from those directed against the GST tag
Reusable – bound antibodies can be eluted with 6M guanidinium chloride allowing for 10 purifications with each column or aliquot of resin
Two formats – choose either pre-packed columns or resin slurry for use at a variety of scales
|Pierce GST Agarose|
|Glutathione S-transferase on crosslinked 6% beaded agarose, slurried in water with sodium azide|
|Binding approx. 0.5mg anti-GST antibody per mL of resin|