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Thermo Scientific™ Pierce™ Coomassie Plus (Bradford) Assay Kit

Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Manufacturer:  Thermo Scientific™ PI23236

Catalog No. PI23236



Thermo Scientific™ Pierce Coomassie Plus Protein Assay is a high-performance Bradford assay reagent — one of the fastest, simplest and most popular protein quantitation methods available.

The Pierce Coomassie Plus Assay Reagent is a single, ready-to-use solution for measuring protein concentration. Simply add the reagent to equal volumes of samples and standards, mix, then measure the absorbance at 595nm. The assay costs only pennies per sample and can be performed in either test tube or microplate format. The Pierce Coomassie Plus Assay Reagent provides increased linearity of response and only half the protein-to-protein variability of other commercial Bradford assay formulations.


  • Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)

  • Easy to use – single reagent; no working reagent preparation required

  • Fast – almost immediate color development; add, mix, and read results

  • Broad range – detects protein concentration in the range 1 to 1500μg/mL

  • Better – improved linearity and response-uniformity compared to traditional Bradford formulations

  • Flexible – microplate and cuvet protocols provided with the instructions and adaptable to several target working ranges


Kit contains Protein Assay Reagent and 10 ampules of Albumin Standard



Pierce Coomassie Plus (Bradford) Protein Assay Kit
Protein Quantitation Assay
Sufficient For: 630 tube assays or 3160 microplate assays
• Pierce Coomassie Plus Assay Reagent, 950 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL

Store Coomassie Plus Assay Reagent at 4°C. Store unopened Albumin Standard Ampules at room temperature.
Not Target-Specific
Solution-based Detection, Absorbance
Spectrophotometer, Microplate Reader