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Thermo Scientific™ Pierce™ Bradford Plus Protein Assay Reagent

Catalog No. PI23238
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300 mL
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PI23238 300 mL
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Catalog No. PI23238 Supplier Thermo Scientific™ Supplier No. LSG23238
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Description

Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce Coomassie Plus Protein Assay is a high-performance Bradford assay reagent — one of the fastest, simplest and most popular protein quantitation methods available.

The Pierce Coomassie Plus Assay Reagent is a single, ready-to-use solution for measuring protein concentration. Simply add the reagent to equal volumes of samples and standards, mix, then measure the absorbance at 595nm. The assay costs only pennies per sample and can be performed in either test tube or microplate format. The Pierce Coomassie Plus Assay Reagent provides increased linearity of response and only half the protein-to-protein variability of other commercial Bradford assay formulations.

Highlights:

  • Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)

  • Easy to use – single reagent; no working reagent preparation required

  • Fast – almost immediate color development; add, mix, and read results

  • Broad range – detects protein concentration in the range 1 to 1500μg/mL

  • Better – improved linearity and response-uniformity compared to traditional Bradford formulations

  • Flexible – microplate and cuvette protocols provided with the instructions and adaptable to several target working ranges

 

Specifications

Content And Storage Store at 4°C.
Description Pierce Bradford Plus Protein Assay Reagent
Assay Bradford Assay
Detection Method Colorimetric
Product Line Pierce
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 300 mL

Frequently Asked Questions (FAQs)

What is Pierce Bradford Plus Protein Assay Reagent?

Pierce Bradford Plus Protein Assay Reagent is a quick and ready-to-use modification of the well-known Bradford, Coomassie dye-binding, colorimetric method for total protein quantitation. This Bradford reagent modification greatly reduces the tendency of Coomassie dye-containing reagents to give a characteristically nonlinear response curve. Pierce Bradford Plus Protein Assay Reagent results in a substantially more linear response curve in a defined range of protein concentration. Thermo Scientific's special formulation results in significantly less protein-to-protein variation than that which is observed with other Bradford-type Coomassie dye-based formulations. When Coomassie dye binds protein in an acidic medium, an immediate absorbance shift occurs from 465 nm to 595 nm, with a simultaneous color change of the reagent from green/brown (or red/brown) to blue.
How can I remove interfering substances from my sample before using the Pierce Bradford Plus Protein Assay Reagent?

You can remove interfering substances easily with the use of one of several Thermo Scientific products designed to purify your sample. These products include Slide-A-Lyzer Dialysis Cassettes, D-Salt Desalting Columns and Extracti-Gel D Detergent Removing Gel. Furthermore, TCA and cold acetone can be utilized to remove other interfering substances. (See the TechTips section of this website for more information.)
Can detergents be used to clean my glassware interfere with the Pierce Bradford Plus Protein Assay Reagent?

Care must be exercised when cleaning glassware that will be used again for protein assays. Thorough cleaning often requires the use of a detergent but it must be completely removed in the final rinse. Also, the Coomassie dye will stain glass or quartz cuvettes. The cuvettes can be cleaned easily with our detergents followed by a thorough final rinsing with deionized water. If you prefer, disposable polystyrene cuvettes may be used to eliminate the cuvette-cleaning chore.
Can I read at wavelengths other than 595 nm with the Pierce Bradford Plus Protein Assay Reagent?

If a photometer or plate reader is not available with a 595 nm filter, the blue color may be measured at any wavelength between 570 nm and 610 nm. The maximum sensitivity of the assay occurs when the absorbance of the Coomassie dye protein complex is measured at 595 nm. Taking the absorbance measurements at any wavelength other than 595 nm will result in a lower slope for the standard curve and may increase the minimum detection level for the protocol.

For Research Use Only. Not for use in diagnostic procedures.