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Thermo Scientific™ Pierce™ Conjugate Purification Kit

Gently purify enzyme-conjugated antibodies from unreacted horseradish peroxidase (HRP) or alkaline phosphatase (AP) after labeling.

Manufacturer:  Thermo Scientific™ 0044920

Catalog No. PI44920



Description

Description

Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.

The metal chelate ligand on the column binds the enzyme conjugates while allowing free enzyme to pass through.

Highlights:

  • Reduced background for blotting and ELISA
  • Fewer washes needed to remove background
  • Increased sensitivity of assay system with more active reagent
  • Improved signal:noise ratio of conjugate

Includes:

Immobilized Iminodiacetic acid 1 × 2mL column, Polyacrylamide Desalting Column 1 × 10mL column, FreeZyme Column Activator Buffer 25mL, FreeZyme Elution Buffer 35mL, BupH™ Tris Buffered Saline Pack two each, BupH Phosphate Buffered Saline Pack one each, Activity Preservation Solution 1mL

Specifications

Specifications

Conjugate Purification Kit
Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
  • IDA Agarose Column, 2 mL, 1 column
  • Polyacrylamide Desalting Column, 10 mL, 1 column
  • Column Activator Buffer, 25 mL
  • Elution Buffer, 35 mL
  • TBS Packs (each makes 500 mL), 2 packs
  • PBS Pack (makes 500 mL), 1 pack
  • Activity Preservation Solution, 1 mL

    Store kit at 4°C.
  • Pierce™
    Antibodies (IgG)
    10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
    0.5 to 3 mg
    HRP (Horseradish Peroxidase)
    SDS
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