Thermo Scientific™ Pierce™ Conjugate Purification Kit
Gently purify enzyme-conjugated antibodies from unreacted horseradish peroxidase (HRP) or alkaline phosphatase (AP) after labeling.
Manufacturer: Thermo Scientific™ 0044920
Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.The metal chelate ligand on the column binds the enzyme conjugates while allowing free enzyme to pass through.
- Reduced background for blotting and ELISA
- Fewer washes needed to remove background
- Increased sensitivity of assay system with more active reagent
- Improved signal:noise ratio of conjugate
Immobilized Iminodiacetic acid 1 × 2mL column, Polyacrylamide Desalting Column 1 × 10mL column, FreeZyme Column Activator Buffer 25mL, FreeZyme Elution Buffer 35mL, BupH™ Tris Buffered Saline Pack two each, BupH Phosphate Buffered Saline Pack one each, Activity Preservation Solution 1mL
|Conjugate Purification Kit|
|Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
Store kit at 4°C.
|10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody|
|0.5 to 3 mg|
|HRP (Horseradish Peroxidase)|