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Thermo Scientific™ Pierce™ Conjugate Purification Kit

Catalog No. PI44920
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Quantity:
1 Kit
Catalog No. Quantity
PI44920 1 Kit
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Catalog No. PI44920

Supplier: Thermo Scientific™ 44920

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Description

Gently purify enzyme-conjugated antibodies from unreacted horseradish peroxidase (HRP) or alkaline phosphatase (AP) after labeling.

Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.

The metal chelate ligand on the column binds the enzyme conjugates while allowing free enzyme to pass through.

Highlights:

  • Reduced background for blotting and ELISA
  • Fewer washes needed to remove background
  • Increased sensitivity of assay system with more active reagent
  • Improved signal:noise ratio of conjugate

Includes:

Immobilized Iminodiacetic acid 1 × 2mL column, Polyacrylamide Desalting Column 1 × 10mL column, FreeZyme Column Activator Buffer 25mL, FreeZyme Elution Buffer 35mL, BupH™ Tris Buffered Saline Pack two each, BupH Phosphate Buffered Saline Pack one each, Activity Preservation Solution 1mL

Specifications

Product Type Conjugate Purification Kit
Quantity 1 Kit
Sufficient For 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
Content And Storage Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
  • IDA Agarose Column, 2 mL, 1 column
  • Polyacrylamide Desalting Column, 10 mL, 1 column
  • Column Activator Buffer, 25 mL
  • Elution Buffer, 35 mL
  • TBS Packs (each makes 500 mL), 2 packs
  • PBS Pack (makes 500 mL), 1 pack
  • Activity Preservation Solution, 1 mL

    Store kit at 4°C.
    		•
    Labeling Scale 0.5 to 3 mg
    Product Line Pierce
    Label or Dye HRP (Horseradish Peroxidase)
    Labeling Target IgG

    For Research Use Only. Not for use in diagnostic procedures.